| Ain't no stoppin'! (Silver) | Defeat 1000 enemies |
| Amazing! (Bronze) | Complete 4 levels with the highrest rank in Hard mode |
| Bug collector (Bronze) | Collect the Hidden Spiders |
| Canceled! (Bronze) | Defeat Deadpool on any difficulty level |
| Clipped Wings (Bronze) | Defeat Hobgoblin on any difficulty level |
| Close call! (Bronze) | Recover 10 times from a Critical Fall |
| Does whatever a spider can! (Platinum) | Unlock all 42 trophies to earn this. |
| Easy as pie! (Bronze) | Complete all levels on Easy |
| End of Act 1 (Bronze) | Complete Act 1 on any difficulty level |
| End of Act 2 (Bronze) | Complete Act 2 on any difficulty level |
| End of Act 3 (Bronze) | Complete Act 3 on any difficulty level |
| Enthusiast (Silver) | Collect 250 Spider Emblems |
| Fanatic (Gold) | Collect 500 Spider Emblems |
| Final curtain call (Silver) | Defeat Mysterio on any difficulty level |
| Freak Show (Bronze) | Defeat Goblin on any difficulty level |
| Getting warmed up (Bronze) | Defeat 100 enemies |
| Hard Pressed (Gold) | Complete all levels on Hard |
| Here's Mud in your Eye! (Bronze) | Defeat Sandman on any difficulty level |
| Hobbyist (Bronze) | Collect 125 Spider Emblems |
| In the zone (Silver) | Defeat 500 enemies |
| Is this normal? (Silver) | Complete all levels on Normal |
| Lay Down Your Arms (Bronze) | Defeat Doctor Octopus on any difficulty level |
| Lead on, M-Dubs! (Bronze) | Complete the Tutorial |
| Manifest Destiny (Gold) | Complete the Web of Destiny |
| Minimized Carnage (Bronze) | Defeat Carnage on any difficulty level |
| Missed me! (Bronze) | Defeat a boss on any difficulty level without taking damage |
| No Harm Done (Silver) | Complete any level without dying (except the Tutorial) |
| Now There's a Shock (Bronze) | Defeat Electro on any difficulty level |
| Sensational!! (Silver) | Complete 8 levels with the highest rank in Hard Mode |
| Smooth moves (Bronze) | Unlock all Combat Upgrades |
| Something CAN Stop Juggernaut (Bronze) | Defeat Juggernaut on any difficulty level |
| Spectacular!!! (Gold) | Complete 12 levels with the highest rank in Hard mode |
| Survival of the Fittest (Bronze) | Defeat Kraven on any difficulty level |
| The Caged Bird Squawks (Bronze) | Defeat Vulture on any difficulty level |
| The complete package (Bronze) | Unlock all Character Upgrades |
| The Hammer Falls (Bronze) | Defeat Hammerhead on any difficult level |
| The Spider's bite (Bronze) | Keep Rage Mode active for one minute |
| The Spider's grace (Bronze) | Complete a 2099 freefall section without taking damage |
| The Spider's shadow (Bronze) | Complete a Noir level without triggering an alarm |
| The Spider's web (Bronze) | Defeat 50 enemies using the Amazing Charge Attack |
| Took The Sting Out of Him (Bronze) | Defeat Scorpion on any difficulty level |
| Two hundo (Bronze) | Execute a 200-hit combo |
| Uncle Benjamin (Bronze) | Execute a 100-hit combo |
