| A Good Dam Fight (25) | Defeat the Battlelord on the Hoover Dam |
| Air-Duke (30) | Win air hockey with a score of 7-0 in the strip club |
| Annihilation (40) | Kill 100 aliens |
| Balls of Steel (25) | Earn a 1,000,000 pinball score in SP |
| Baron von Nukem (20) | Shoot down 20 alien fighters |
| Beating the One-Eyed Worm (35) | Defeat the Energy Leech |
| Big Guns, Big Ships (10) | Blow up 5 enemy gunships or dropships |
| Bucket Head (30) | Find all 3 helmets in the SP campaign |
| Call Waiting (25) | Listen to all phone messages |
| Come Get Some (40) | Complete the SP campaign on Hard Difficulty |
| Companion Barrel (10) | Unlock the secret closet at the end of the Forkstop |
| Damn, I'm Good (50) | Complete the SP campaign on Insane Difficulty |
| Dead Useful (15) | Kill 10 aliens with environmental explosives |
| Downtown Barrel Beatdown (15) | Kill 10 aliens with tossed objects |
| Drawrings (10) | Doodle something on the whiteboard in SP |
| Duke Angry, Duke Smash! (20) | Kill 15 aliens with melee attacks while on steroids |
| Extermination (25) | Kill 50 aliens |
| FBT (10) | Get knocked down 10 times |
| Flagon of Chuckles (5) | Drink a beer in SP |
| Fork the Pork (10) | Kill 6 aliens with the forklift |
| Freeze Well! (20) | Kill 15 frozen aliens |
| Gunslinger (50) | Carry the gold pistol through the whole SP campaign |
| He's Got a Hologram! (5) | Use a Holoduke in SP |
| Hippy-Stomper (20) | Foot stomp 12 aliens |
| I Am All That Is Man (40) | Discover all Ego cap awards |
| I Need a Date (5) | Look at every page of a calendar in SP |
| I Need a Towel (10) | Get hit by 10 Pregnator bombs |
| Judge, Jury, Executioner (20) | Execute 20 aliens |
| Juiced (5) | Take steroids in SP |
| Let's Rock (30) | Complete the SP campaign on Normal Difficulty |
| Lots of Whacking (25) | Win a game of Alien Abortion in the strip club |
| Natural Disaster 3x (10) | Kill 3 aliens at once |
| Nobody Likes a Whiner (5) | Knock out the talent at the talk show |
| Noms (10) | Eat 10 pieces of food during the SP campaign |
| Not Bad for a Human (40) | Defeat the Alien Queen |
| Nuclear Devastation (50) | Kill 250 aliens |
| Octacide (25) | Defeat the Octaking |
| On the Noggin (25) | Kill 30 aliens with headshots |
| One-Eyed Freak (10) | Defeat the Cycloid |
| Party Animal (10) | Drink all of the beers in the strip club |
| Pescaphobe (10) | Kill all the catfish in the underwater level |
| Piece of Cake (20) | Complete the SP campaign on Easy Difficulty |
| Pit Champion (25) | Defeat the Battlelord in Las Vegas |
| Road Rage (20) | Kill 15 aliens with the monster truck |
| Special Thanks (5) | Watch the credits all the way through |
| Sticky Bomb Like You! (10) | Put a Trip Mine on a live alien |
| Substance Abuser (10) | Drink beer while on steroids or vice versa in SP |
| Sunday, Black Sunday (5) | Shoot down the blimp above the stadium |
| Trapper (20) | Kill 10 aliens with Trip Mines |
| Turd Burglar (10) | Find and steal a piece of poo |
